Figure 8.

Secretion of Pmel17 is up-regulated by PMA, NaN₃, or W-7, but is not inhibited by metal chelators. A) MNT-1 cells were immunocytochemically stained with HMB50 in the presence or absence of TX. Scale bar = 20 μm. B–D) MNT-1 cells were radiolabeled and chased in the presence of 200 nM PMA, DMSO, 20 mM NaN₃, 5 mM EGTA, and 5 mM EDTA (B), 50 μM W-7 (C), or 100 nM GM-6001 and 10 μg/ml BFA (D) for 4 h. One sample was chased at 4°C. Medium was analyzed as described previously. Intensities of specific bands, Mα, relative to control are quantified (bottom panel). Graphed data are means ± sd from 3 independent experiments.