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. 2010 Mar;24(3):833–843. doi: 10.1096/fj.09-142976

Figure 3.

Figure 3.

Hypophosphorylated RARα enhances transcriptional activity while diminishing binding to RARE. A) EMSA analysis of RA-induced interaction between nuclear protein and hβRARE. NE, nuclear extracts isolated from F9 cells. B) EMSA supershift assay of specific binding of nuclear RARα/RXRα to hβRARE. C) EMSA analysis of the effect of CAK phosphorylation of RARα on RARα-hβRARE interaction. cCAK, cellular CAK immunoprecipitated from F9 cells. D) EMSA and EMSA supershift analyses of specific interactions between hβRARE and cellular S77A or RARα expressed in F9 RARα−/− cells. E) Luciferase reporter assay of transcriptional activities in F9 cells treated with ATRA. F) S77A expressed in F9 RARα−/− cells enhances luciferase activity in the absence of ATRA. *P < 0.005 vs. other groups. G) S77A expressed in F9 RARα−/− cells enhances luciferase activity in the presence or absence of ATRA. *P < 0.003, **P < 0.001 vs. vector; #P < 0.02 vs. RARα and vector.