Figure 5.

Increased lipase activity in the supernatant of H₂O₂-treated B. cenocepacia J2315 biofilms. Lipase activity was determined in the supernatant of B. cenocepacia J2315 biofilms that were either untreated (diamonds) or that were treated with 0.3% H₂O₂ for 15 (rectangles), 30 (triangles) or 60 min (circles). The graph shows a representative example of the obtained curves using 4-MU oleate as fluorogenic substrate. Signals are expressed as normalized fluorescence units (RFU). Error bars represent standard deviations.