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. Author manuscript; available in PMC: 2011 Feb 17.
Published in final edited form as: Cancer Cell. 2010 Feb 17;17(2):148–159. doi: 10.1016/j.ccr.2009.12.034

Fig. 4. Wt Mll excision reduces the number of MA9-transformed cells and Hox gene expression.

Fig. 4

(A) A diagram for the floxed Mll and the primers used to detect the intact or excised Mll. (B) 4-OHT induced excision of the floxed Mll. Spleen cells from a Mllf/f;Cre-ER mouse were cultured with either DMSO or 4-OHT, followed by isolation of the genomic DNA and PCR amplification. (C) A growth curve for MA9-transformed BM cells with either Mllf/f or MllΔ/Δ (+/− SD, cells seeded in triplicate) (D) Quantification of Hoxa9 and Hoxa5 mRNAs in either Mllf/f or MllΔ/Δ MA9-transformed cells (+/− SD). See also Figure S3.