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. Author manuscript; available in PMC: 2011 Feb 17.
Published in final edited form as: Cancer Cell. 2010 Feb 17;17(2):148–159. doi: 10.1016/j.ccr.2009.12.034

Figure 6. Wt MLL knockdown suppresses colony formation of MA9-transduced BM.

Figure 6

(A) Procedure for the colony formation assay. Bone marrow (BM) cells from a C57B6 mouse were transduced with pMSCV-MA9 retroviruses, and replated in triplicate weekly in methylcellulose medium with G418. After the second plating, surviving MA9 cells were transduced with each of the MLL-C shRNAs (12 and 14) or scrambled vector. (B) A summary of colony numbers for control or Mll shRNA-transduced BM. (C) Representative colonies from the culture plates (Scale bars 5mM). Error bars denote +/− SD.