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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Mol Immunol. 2009 Dec 30;47(6):1356–1365. doi: 10.1016/j.molimm.2009.12.003

Fig. 2. Transient expression of CHOP during B lymphocyte differentiation.

Fig. 2

A) CD19+ B splenocytes from wt and chop-/- mice were cultured in vitro with 20 μg/ml LPS for the indicated time points. chop mRNA expression pattern during differentiation was followed by relative real time PCR as in Fig.1 C.

B) Nuclear accumulation of CHOP protein in B splenocytes, differentiated as in panel A, was followed by Western blot analyses. When indicated, cells were treated with 2 μg/ml thapsigargin (Th) for 3 hrs. Ponceau staining of a region of the nitrocellulose filter is shown as loading control.

C) CHOP is transiently induced also in in vivo differentiating B cells. c57/BL6 mice were injected intra-peritoneally with LPS. After LPS injection, 3 mice were sacrificed at each time point and differentiating B cells were purified and mixed to extract total RNA. CHOP transcript was detected as in panel A.