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. Author manuscript; available in PMC: 2011 Apr 15.
Published in final edited form as: Anal Biochem. 2009 Dec 28;399(2):182–189. doi: 10.1016/j.ab.2009.12.027

Figure 3.

Figure 3

(a) Representative fluorescence spectra of HP35-AP obtained with λex = 240 nm and at different urea concentrations, as indicated. (b) Normalized fluorescence intensities of the donor (PheCN) and acceptor (7AW) versus urea concentration, as indicated. These data correspond to the maximum values of the respective PheCN and 7AW fluorescence emissions in the FRET spectra, e.g., those in (a). Also shown (open circle) is the normalized fluorescence intensity of 7AW obtained with λex = 310 nm using the same sample as that used in the corresponding FRET measurement.