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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Mol Immunol. 2010 Jan 18;47(6):1262–1268. doi: 10.1016/j.molimm.2009.12.010

Figure 5. Ectopic expression of RORγt enhances IL-17 expression in T-CD4 T cells.

Figure 5

Freshly isolated CD4 T cells from both WT and Tg mice were cultured under Th17 inducing conditions for different time points and RNA was extracted and analyzed for IL-17 (A) and RORγt (B) by qRT-PCR. (C) CD4 T cells from WT and Tg mice were used for the ChIP assay with an anti-acetylated histone H3 antibody. PCR primers specific for the IL-17 enhancer were used to amplify the precipitated DNA Primers specific for CD3ε were used as an internal loading control. (D) WT and Tg CD4 T cells were transduced with viruses encoding IRES-GFP (MIG) or RORγt-IRES-GFP (RORγt) under Th17 inducing condition. Cells were analyzed after 5 days in culture. Data shown are representative of at least three independent experiments.