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. Author manuscript; available in PMC: 2011 Feb 26.
Published in final edited form as: Immunity. 2010 Feb 11;32(2):253–265. doi: 10.1016/j.immuni.2010.01.010

Figure 1. Integrin-mediated adhesion is critical for initial but not prolonged T:B cell conjugation.

Figure 1

(A–B) Adhesion of pre-activated WT and Sh2d1a−/− CD4+ T cells to recombinant ICAM2-Fc following α-CD3 stimulation (A) 10 min. and (B) 15–60 min. Results presented as the mean percent adhesion (A) n=3 ± SEM. (B) n=6 ± SEM. (C–D) Conjugation efficiency after pre-incubation with blocking integrin antibodies then incubation for 10–30 min with OVA323 pulsed B cells (C), mean ± SEM frequency of CD4+CD19+ conjugates in total CD4+ events (n=3) or (D) DCs, mean ± SEM frequency of CD4+CD11c+ conjugates in total CD4+ events (n=3). **p<0.005.