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. Author manuscript; available in PMC: 2011 Feb 26.
Published in final edited form as: Immunity. 2010 Feb 11;32(2):253–265. doi: 10.1016/j.immuni.2010.01.010

Figure 2. Importance of SAP in T:B adhesion is consistent with SLAM family member expression on activated B cells.

Figure 2

(A) OT-II T:B cell conjugation assays were conducted using CD4+ T cells transiently transfected with DNA constructs expressing either GFP, GFP-SAP, GFP-SAP(R78A), or GFP-SAP(R55L), n=6, mean ± SEM frequency of CD4+CD19+ conjugates in total GFP+CD4+ events, **p<0.005 (See Figure S2 for western blot and representative FACS plot). (B) CD11c+ splenic DCs, splenic B cells and LPS-activated B cells were assessed for SLAM, Ly108, and CD84 expression (n=3). (C–D) Day 9 post-NP-OVA in alum immunization, (C) B cells or (D) Tfh (CD4+CD44+CXCR5hiPD-1hi) and non-Tfh (CD4+CD44+CXCR5loPD-1lo) cells were evaluated for Ly108 and CD84 expression (n=2, 2–5 mice/genotype).