Fig. 8.
Electron micrograph after the IPTG blockage release.
BSC40 cells were infected with vE6i at moi = 10 pfu/cell and incubated in absence IPTG. At 12 hpi, fresh media with no drugs (A, B) or containing IPTG (C, D) or IPTG + CAR (E, F) were added to the cells. After 24h of the blockage release (36 hpi), the cells were processed for microscopy as described in Methods. EIV = empty immature virions; IV= immature virions; MV= mature virions; WV= wrapped virions; C= crescents; RAV = rough aggregated virosome; SAV = smooth aggregated virosome; N=nucleus.