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. 2010 Feb;30(2):67–79. doi: 10.1089/jir.2009.0046

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© Mary Ann Liebert, Inc.

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FIG. 8. — Interleukin-7 receptor (IL-7R) and CD103 expression are shown as measured by mRNA and flow cytometric analysis. (A) IL-7R mRNA expression on sorted CD4+ IEL. Note an increase in IL-7R in the total parenteral nutrition (TPN) group, and a slightly lower amount of increase in the TPN+glutamine (TPN+GLN) group. Differences were not significant. (B) IL-7R mRNA expression on sorted CD8+ IEL. Note a significant increase in IL-7R in the TPN group, and a sustained increased expression of IL-7R in the TPN+GLN group. (C) Epithelial cell CD103 mRNA expression. Note a significantly decreased expression in the TPN group, and a prevention of this decline in the TPN+GLN group. (D) Flow cytometric analysis of gated IEL and EC populations. Surface expression of IL-7R is given for both the CD4+ and CD8+ IEL populations. Although IL-7R increased with TPN, and CD103 declined, the changes were not significantly different. Values are expressed as the percent of gated cells positive for each target population. *P < 0.05 vs. control; #P < 0.05 vs. TPN group.