Skip to main content
. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Am J Med Genet A. 2010 Mar;152A(3):646–652. doi: 10.1002/ajmg.a.33299

Figure 2. Radixin is not targeted by miR-96 and miR-182 in vitro.

Figure 2

Figure 2

A Luciferase reporter activity of reference (*95C) and mutant (*95A) RDX 3′UTR constructs co-transfected with miRNA mimics in HEK293 cells. Blue bars = reference sequence; red bars = mutant sequence. Data are averages of six replicates. Error bars denote standard deviation (SD). B Western blot analysis of endogenous radixin protein levels in HeLa cells treated with miRNA mimics (20, 200 pmol). miR-31 was included based on similarity to miR-96 and -182. miR Pool denotes cocktail of all three miR mimics. Cell lysates were collected 72 hrs after transfection. Blots were stripped and reprobed with alpha-tubulin antibody as a loading control.