Figure 6.

Dye coupling in OECs. A, Cluster of 11 dye-coupled OECs (green) with nuclei stained with DRAQ5 (blue). Grid: 10 μm. Arrow in A and B: recorded cell. GL: glomerular layer. Dashed lines delineate the glomeruli. Asterisks in B: nuclei of dye-coupled cells. C, Isosurface rendering of the green channel. The nuclear staining (blue channel) was used to generate contour surfaces of the nuclei of the injected cell (blue) and dye-coupled cells (purple). D, Segments: linear distance between the cell body of the injected cell and those of the coupled cells (red) and cluster main axis (blue, see Materials and methods). Inset: the same cluster oriented with north (N) pointing up and the equator indicated (dashed yellow line). E, Proportion of coupled cells vs. the proportion of nuclei in the northern hemisphere (N). Each point is a different cluster. The vertical and horizontal dotted lines correspond to symmetrical distribution of nuclei and coupled cells on both sides of the equator, respectively. The dashed line corresponds to slope=1. F, Dye intensity at the cell bodies of coupled cells vs. the linear distance to the injected cell. Each point is a different cell and each color is a different cluster, matching E. The lines in E and F are linear regressions.