Table 2.

Assignment of tryptic peptide sequences and reported CCS values.

Protein	Observed [M + H]+a	[M + H]+calc	Δppm	Sequenceb	CCS (Å2)c
actin	1198.70	1198.706	−5.0	AVFPSIVGRPR	282.0 ± 2.2
	1515.74	1515.7497	−6.4	IWHHTFYNELR	323.93 ± 2.8
myelin basic protein	709.34	709.3422	−3.1	FSWGGR	193.9 ± 2.4
	726.40	726.4051	−7.0	HGFLPR	203.4 ± 2.2
	1131.57	1131.5798	−8.6	TTHYGSLPQK	267.4 ± 2.0
	1336.63	1336.6319	−1.4	YLATASTMDHAR	296.3 ± 2.5
	1339.70	1339.7082	−6.1	HRDTGILDSIGR	288.8 ± 1.9
	1460.71	1460.7174	−5.0	TQDENPVVHFFK	310.2 ± 1.7
	1800.84	1800.8458	−3.2	FSWGAEGQKPGFGYGGR	348.2 ± 2.8
neurofilament protein	1265.65	1265.6563	−4.9	MALDIEIAAYR	294.7 ± 5.4
	1296.70	1296.5682	−6.3	NMQNAEEWFK	291.6 ± 2.1
tubulin, alpha	1701.90	1701.9063	−3.7	AVFVDLEPTVIDEVR	356.1 ± 1.7
	1807.87	1807.8834	−7.4	AVCMLSNTTAIAEAWAR	385.7 ± 2.2
	2006.89	2007.8936	−1.7	TIGGGDDSFNTFFSETGAGK	386.9 ± 3.1
tubulin, beta	971.49	971.4984	−8.6	TAVCDIPPR	241.5 ± 1.5
	1039.59	1039.594	−3.8	YLTVAAVFR	276.3 ± 1.5
	1053.60	1053.6097	−9.2	YLTVAAIFR	277.7 ± 1.7
	1077.53	1077.5328	−2.5	IREEYPDR	252.5 ± 2.2
	1143.63	1143.6348	−4.1	LAVNMVPFPR	275.0 ± 2.5
	1229.59	1229.5988	−7.1	ISEQFTAMFR	283.1 ± 1.7
	1258.68	1258.6908	−8.5	FPGQLNADLRK	287.9 ± 1.9
	1271.72	1271.7298	−7.7	KLAVNMVPFPR	293.6 ± 1.8
	1601.81	1601.8209	−6.8	AVLVDLEPGTMDSVR	333.0 ± 2.4
	1615.83	1615.8365	−4.0	AILVDLEPGTMDSVR	336.2 ± 2.7
	1620.83	1620.8361	−3.7	LHFFMPGFAPLTSR	345.9 ± 2.2

^aData acquired by MALDI-FTICR mass spectrometer.

^bMALDI MS/MS analysis of tryptic peptides directly from tissue sections acquired by MALDI Q-TOFMS.

^cCCS values are reported as the average of 10 replicate measurements using BSA tryptic peptides as external calibrants (reported in table S2).