Table 3.
Mammalian Species | Protein | Tryptic Peptide Sequence | m/z | Drift Cell IM-MS CCS (Å2)a | T-Wave IM-MS CCS (Å2)b |
---|---|---|---|---|---|
Mouse | Myelin Basic Protein | FSWGGR | 709.3 | 193.7 ± 2.8 | 193.9 ± 2.3 |
HGFLRP | 726.4 | 204.7 ± 3.1 | 203.4 ± 2.2 | ||
YLATASTMDHAR | 1336.6 | 289.5 ± 9.5 | 296.4 ± 2.5 | ||
HRDTGILDSIGR | 1339.7 | 283.1 ± 8.0 | 288.9 ± 2.0 | ||
TQDENPVVHFFK | 1460.7 | 305.5 ± 8.0 | 310.3 ± 1.8 | ||
Bovine | Myelin Basic Protein | HGFLRP | 726.4 | 205.0 ± 2.3 | 203.4 ± 2.2 |
HRDTGILDSIGR | 1339.7 | 286.1 ± 8.1 | 288.9 ± 2.0 | ||
Actin | AVFPSIVGRPR | 1198.7 | 279.5 ± 6.8 | 282.0 ± 2.3 | |
IWHHTFYNELR | 1515.9 | 320.9 ± 7.7 | 323.9 ± 2.9 |
CCS values are reported as the average of 10 replicate measurements.
CCS values are reported as the average of 10 replicate measurements using external BSA tryptic peptides as calibrants.