Skip to main content
. Author manuscript; available in PMC: 2011 Apr 10.
Published in final edited form as: Virology. 2010 Feb 8;399(2):221–230. doi: 10.1016/j.virol.2010.01.010

Fig. 1. One-step growth and DNA replication in cells infected with wt and mutants in the E6R gene.

Fig. 1

A) BSC40 cells were infected at moi = 10 pfu/cell with wt (VACV-WR) or Cts52 incubated at 31 or 40°C. At indicated times post-infection samples were removed and virus yields were determined by plaque titration at 31°C. B) BSC40 cells were infected wt (VACV-IHD-W), or Dts41or Dts80 as described above. Virus yield was determined by plaque assay at 31°C after 24 and 48 hpi. C) BSC-40 cells were infected wt (VACV-WR) or Cts52, incubated at 31 or 40 °C as described above. At indicated times post-infection, cell extracts were prepared and applied in triplicate on nylon membranes, and hybridization to a 32P labeled vaccinia virus DNA probe was performed as described under Methods. The numbers (arbitrary units) express the average value of three different samples. wt-31°C (◆), wt-40 °C (∎), Cts52-31 °C (▴), Cts52-40 °C (●).