BSC40 cells were infected with moi= 1000 particles of wt (A, C, E, G) or Cts52 (B, D, F, H) virus grown at 31 °C (A, B, E, F) or 40 °C (C, D, G, H). The cells were processed and stained for viral membrane protein A27 (green) (A-D) or viral core protein A4 (red) (E-H), and DNA was stained with Dapi (blue). The cells were analyzed by confocal microscopy as described in Methods.