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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Protein Expr Purif. 2010 Jan 4;71(1):62–73. doi: 10.1016/j.pep.2009.12.013

Figure 5. In vitro fluorescent labeling of AcTEV-treated and rEK-treated BoNT/Aadek with Sfp phosphopantetheinyl transferase and CoA 547.

Figure 5

Lanes 1 - 4, unreduced samples; lanes 5 - 8, samples reduced by addition of β-mercaptoethanol. Lanes 1, 3, 5, 7: 0.02 μg BoNT/Aadek; lanes 2, 4, 6, 8: 0.1 μg BoNT/Aadek. Panel A: 10.5 - 14% Criterion gel (Bio-Rad) stained with Bio-Safe Coomassie (Bio-Rad). Panel B: Western blot of gel shown in panel A scanned on a Typhoon 9500 scanner (GE Healthcare) using 300V PMT, 532/580 nm excitation/emission filter set (green).