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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Antiviral Res. 2009 Dec 5;85(3):482–489. doi: 10.1016/j.antiviral.2009.12.003

Fig. 3.

Fig. 3

Purified recombinant APP-S. Recombinant human APP-S was expressed in BL21-RIPL cells and purified using a Ni-NTA affinity column. APP-S was eluted via cleavage by thrombin between the His-tag and the recombinant APP-S. Following quantification of total protein using the BCA assay (Pierce), 80 ng of APP-S was run in a 4-12% Bis-Tris gel (Invitrogen) and stained with Krypton Protein Stain (Pierce Biotechnology, Inc.). Lane 1 contains BenchMark Protein Ladder (Invitrogen) and lane 2 contains the purified recombinant APP-S, which was purified to > 97% purity as determined by ImageQuant Analysis (Molecular Dynamics).