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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Antiviral Res. 2009 Dec 5;85(3):482–489. doi: 10.1016/j.antiviral.2009.12.003

Fig. 5.

Fig. 5

Chemical structures of the observed activating metabolites obtained during the hydrolysis of Val-Ser-cHPMPC by recombinant APP-S. Samples from APP-S hydrolysis of Val-Ser-cHPMPC were analyzed by LC/MS. It was found that the peak corresponding to 3 was present in the samples hydrolyzed by APP-S, but not present in the negative control samples (prodrug in buffer alone or with APP-S and bestatin). Enzymatic (APP-S), as well as chemical hydrolysis, is involved in the overall prodrug activation process.