Table I.
Purification step | Total protein | Specific activity | Half-life of Val-Ser-cHPMPC | Purification |
---|---|---|---|---|
mg | nmol/min·mg protein | min | -fold | |
Cytosolic fraction | 174 | 6.8 | 13.6 | 1.0 |
(NH4)2SO4 precipitation | 55 | 11.2 | 7.2 | 1.7 |
Bestatin column | 5.1 | ND a | ND | ND |
MonoQ column | 0.8 | 142.5 | 4.6 | 22 |
ND = not determined