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. Author manuscript; available in PMC: 2011 Feb 26.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Jan 25;393(1):66–72. doi: 10.1016/j.bbrc.2010.01.080

Figure 1. Effect of CSE and H2O2 on SIRT1 levels and activity in HUVECs.

Figure 1

Figure 1

SIRT1 levels: A) HUVECs were treated with CSE (0.5% and 1.0%) and H2O2 (25, 50 and 100 μM) for indicated time points. B) and C) Histograms represent the relative intensity of SIRT1 levels (bands) compared to respective controls (n=3). SIRT1 activity: D) HUVECs were treated with CSE (1.0%) and H2O2 (100 μM) for 1 hr, and at 4 hr alone or in combination with pretreatment of resveratrol (10 μM) and splitomicin (10 μM), and SIRT1 deacetylase activity was determined. Data are Mean ± SE (n=3). *P < 0.05, **P < 0.01, ***P < 0.001 vs. controls.