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BALF cytokine level and IL-10–secreting CD4+CD25+ T-regulatory cells. BALF samples were immediately centrifuged and level of cytokines, IL-5 (A), IFNγ (B), and IL-10 (C), in the supernatants was measured. Data are shown as means (±SEM) for six animals in each group (*P < 0.01 and **P < 0.001). (D) CD4+CD25+ T cells were isolated, cultured, and plated on ELISPOT plates to evaluate expression of IL-10. Data are shown as means (±SEM) for five animals in each group (**P < 0.001).
