Figure 7.

(A and B) The effect of Flt3-L on lung CD4⁺CD25⁺ T cells expressing Forkhead winged helix transcription factor box P3 (Foxp3) and GATA3. CD4⁺CD25⁺ T cell isolated from the lungs of PBS control mice showed normal expression of Foxp3 mRNA (480 bp) and no detectable level of GATA3 (570 bp). However, cockroach sensitization induced a significant decrease in Foxp3 expression, but substantially increased GATA3 expression in CD4⁺CD25⁺ T cell isolated from the lung tissue. Flt3-L caused a significant increase in Foxp3 and decreased expression of GATA3 compared with CRA mice and PBS control group. HPRT (660 bp) was used as the housekeeping gene. Densitometric analyses confirmed the PCR data by showing the ratio of mRNA intensity of Foxp3 and GATA3 by dividing by the intensity of the housekeeping gene, HPRT (**P < 0.001).