Table 3.

Fluorescence kinetics at 680 nm of PSII in thylakoids of WT A. thaliana

Wavelengths used for PSI removal	PSII ratios from 251 ps DAS				PSII ratios from 532 ps DAS
	700/680 nm		720/680 nm		700/680 nm		720/680 nm
Excitation	412 nm	484 nm	412 nm	484 nm	412 nm	484 nm	412 nm	484 nm
τ (ps)	$p_{\text{PSII}}^{\text{i}}$	$p_{\text{PSII}}^{\text{i}}$	$p_{\text{PSII}}^{\text{i}}$	$p_{\text{PSII}}^{\text{i}}$	$p_{\text{PSII}}^{\text{i}}$	$p_{\text{PSII}}^{\text{i}}$	$p_{\text{PSII}}^{\text{i}}$	$p_{\text{PSII}}^{\text{i}}$
73	0.195	0.122	0.185	0.125	0.187	0.119	0.181	0.125
251	0.424	0.487	0.429	0.485	0.428	0.489	0.431	0.485
532	0.381	0.391	0.386	0.390	0.385	0.392	0.388	0.390
τavg (ps)	323	339	326	338	326	340	328	338

The kinetics were derived from the kinetics of thylakoid membranes by removing the PSI contribution, using the ratios of the PSI and PSII DAS at 700/680 nm or 720/680 nm. The PSI ratios were taken from literature (35–37), and the PSII ratios were obtained from the PSII DAS of either 251 ps or 532 ps. For details, see text. Confidence intervals of fluorescence lifetimes (τ) were <5% (calculated by exhaustive search), standard errors of amplitudes $\left(p_{\text{PSII}}^{\text{i}}\right)$ were generally <5% (calculated from 2–5 repeats). Confidence intervals of average lifetimes (τ_avg) were <1%. This is smaller than the uncertainties of the fit parameters due to correlations between fit parameters.