Table 3.
Wavelengths used for PSI removal | PSII ratios from 251 ps DAS |
PSII ratios from 532 ps DAS |
||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
700/680 nm | 720/680 nm | 700/680 nm | 720/680 nm | |||||||||||||
Excitation | 412 nm | 484 nm | 412 nm | 484 nm | 412 nm | 484 nm | 412 nm | 484 nm | ||||||||
τ (ps) | ||||||||||||||||
73 | 0.195 | 0.122 | 0.185 | 0.125 | 0.187 | 0.119 | 0.181 | 0.125 | ||||||||
251 | 0.424 | 0.487 | 0.429 | 0.485 | 0.428 | 0.489 | 0.431 | 0.485 | ||||||||
532 | 0.381 | 0.391 | 0.386 | 0.390 | 0.385 | 0.392 | 0.388 | 0.390 | ||||||||
τavg (ps) | 323 | 339 | 326 | 338 | 326 | 340 | 328 | 338 |
The kinetics were derived from the kinetics of thylakoid membranes by removing the PSI contribution, using the ratios of the PSI and PSII DAS at 700/680 nm or 720/680 nm. The PSI ratios were taken from literature (35–37), and the PSII ratios were obtained from the PSII DAS of either 251 ps or 532 ps. For details, see text. Confidence intervals of fluorescence lifetimes (τ) were <5% (calculated by exhaustive search), standard errors of amplitudes were generally <5% (calculated from 2–5 repeats). Confidence intervals of average lifetimes (τavg) were <1%. This is smaller than the uncertainties of the fit parameters due to correlations between fit parameters.