Figure 1. Inducible expression of EGFP through the T. congolense life cycle.

Microscope images of the in vitro cultivated IL3000:13–29 strain transfected with pLEW20c-EGFP vector in all the developmental stages. Non induced and tetracycline (1 µg/ml for 48 h) induced cells were fixed and visualized in phase contrast and by EGFP fluorescence (images were recorded with the same exposure time). Scale bars = 1 µm.