Table 1. Parasite culture media.

Code		Basal medium (mM)								Supplements (mM)							serum (% v/v)
	Base powder	Hep	Gluc	Pyr	Ade	Hypo	Thy	Bac	Gln	Pro	2 Me	Cys	Aco	Cit	RBC	FCS	GS	FGS	SP
TcPCF-1	MEM	25				0.1			2	10			3	3		20
TcPCF-2	MEM	25				0.1			2	10			3	3			20
TcPCF-3	MEM	25				0.1			2	10						20
TcEMF-1	MEM								2	10
TcEMF-2	MEM								2	10						10
TcBSF-1	MEM	25	5.5	1	0.04	0.1	0.02	0.02	2		0.2						20		5
TcBSF-2	MEM	25	5.5	1	0.04	0.1	0.02	0.02	2		0.2				0.5		20		5
TcBSF-3	MEM	25	5.5	1	0.04	0.1	0.02	0.02	2		0.2				0.5			20	5

MEM, Eagle's Minimum Essential Medium powder medium (M0643, Sigma-Aldrich); IMDM, Iscove's Modified Dulbecco's Medium (42200014, Invitrogen). Both media were supplemented with 2.2 g of sodium bicarbonate (Sigma-Aldrich). Basal medium components were resuspended in 1 L Versol water (Revol, Villeurbanne) and after adjustment of the pH to 7.3 with 5 N NaOH, the medium was filter sterilized (Millipore 0.22 um), stored at 4°C and used within 10 days. Before use, basal medium was supplemented with the components listed (Sigma-Aldrich) and serum. Hep, HEPES Acid; Gluc, D-Glucose; Pyr, Sodium Pyruvate; Ade, Adenosine; Hypo, Hypoxanthine; Thy, Thymidine; Bac, Bathocuproin; Gln, Glutamine; Pro, Proline; 2 Me, 2-mercaptoethanol; Cys, Cystein; Aco, Cis-aconitate; Cit, Citrate. RBC, Red Blood Cell lysate was obtained after centrifugation (1 min 2000 rpm) of 100 µl of mouse blood, and resuspending of the pellet in 50 µl sterile distilled water. FCS, Heat-inactivated (56°C, 30 min) fetal calf serum (Adgenix); GS, Goat Serum (Invitrogen); FGS, Fresh Goat Serum; SP, Serum Plus (SAFC Biosciences, United Kingdom).