Table 3. In vitro cycle timing and virulence of various T. congolense strains.

Strain	Differenciation timing during metacyclogenesis (weeks)			Virulence of		Mice survival (Days)	Maximum parasitaemia (108/ml)	Minimum PCV (%)
	Mouse>PCF	PCF>EMF	EMF>MCF	MCF	BSF
ILC49	4 to 6	3 to 6	1 to 2	−	acute	3.9±0.3	1.8±0.2	44.7±1.5
IL3000	3 to 4	1 to 3	1 to 2	acute	acute	14.1±1.2	10.2±0.1	28.0±2.6
IL1180	3 to 4	3 to 6	1 to 2	chronic	chronic	48.7±4.6	5.4±0.3	25.3±3.0
TREU 1457	4 to 6	3 to 6	1 to 2	acute	acute	12.1±1.6	5.2±0.2	30.2±2.8
STIB 910	6 to 8	3 to 6	1 to 2	acute	acute	11.9±3.2	10.1±0.4	26.1±1.6
TRUM183:13–29	3 to 4	3 to 6	1 to 2	−	acute	15.2±4.3	5.6±0.3	31.3±2.1
IL3000:13–29	3 to 4	4 to 8	1 to 2	−	acute	13.2±2.6	5.4±0.1	28.6±1.4

Mice survival, Maximum parasitaemia and Minimum PCV were expressed as mean values calculated from experimental infections of at least 5 mice after infection with 10⁷ BSF obtained after at least 5 passages in mice. Timings were the same whether TRUM183:13–29 and IL3000:13–29 were transfected with pLEW20c derivatives or not. MCF were purified from in vitro cultured parasites by DE52 column chromatography, BSF were present in mouse blood after at least 5 passages.