Table 4. Luciferase activity in the T. congolense pLEW20c-Rluc transfected strains.
Strain | Clone | Tetra | Luciferase Activity (RLU/mg of proteins) in the different parasitic stages | ||||
PCF | EMF | MCF | BSFc | BSFb | |||
Treu 183:13–29 pLEW20_LucR | C2 | − | 356±32 | 294±21 | 437±33 | 370±27 | 98±22 |
+ | 1233±26 | 978±24 | 978±36 | 1061±32 | |||
IL3000:13–29 pLEW20_LucR | C6 | − | 235±41 | nd | nd | 300±30 | 136±29 |
+ | 1045±34 | nd | nd | 1288±19 | |||
B1 | − | 288±10 | |||||
+ | 1300±35 | ||||||
IL3000 pLEW20_LucR | B2 | − | 1467±32 |
Tetra: absence (−) or presence (+) of tetracycline (1 µg/ml) in the culture. BSFc: cultivated BSF, BSFb: BSF from infected mice blood. nd: not determined.