Figure 3. Average synonymous versus nonsynonymous nucleotide substitutions in piscidins.

Cumulative nonsynonymous (solid green line) and synonymous (dashed red line) substitutions for all pairwise comparisons are plotted along the first 46 residues of the piscidin precursor. Generally the number of nonsynonymous substitutions exceeds the synonymous replacements throughout the coding sequence. Divergence at nonsynonymous sites is higher in the mature peptide region. dN and dS values for the signal and mature peptides are also indicated above the corresponding regions.