Table 2.
In vitro Phenotypic Characteristics#
| Gene | BMMSCs | SHED | DPSCs* | SCAP* | PDLSCs | DFPCs |
|---|---|---|---|---|---|---|
| STRO-1 | + (5-10%) | + (9%) | + (5-10%) | + (> 18%) | + | + |
| CD + (selected) | CD44 | CD146 | CD13 | CD13 | CD13 | CD13CD29 |
| CD73 | CD29 | CD44 | CD29 | |||
| CD105 | CD44 | CD24 | CD44 | CD44 | ||
| CD106 | CD59 | CD29 | CD59 | CD59 | ||
| CD73 | CD73 | CD90 | CD73 | |||
| CD90 | CD90 | CD105 | CD90 | |||
| CD105 | CD105 | CD105 | ||||
| CD146 | CD106 | |||||
| CD146 | ||||||
| CD- (selected) | CD14 | CD14 | CD18 | CD45 | ||
| CD34 | CD24 | CD34 | ||||
| CD45 | CD34 | CD45 | ||||
| CD45 | CD150 | |||||
| Oct4 | + | + | + | |||
| Nanog | + | + | ||||
| Survivin | *– | *++ | ||||
| hTERT | − | *+ | *++ | |||
| Notch-1 | + | |||||
| TGFβRI | + | + | ||||
| TGFβRII | + | *+ | *+/– | |||
| Endostatin | + | *++ | *++ | |||
| bFGF | + | *++ | *++ | |||
| FGFR3 | + | *+++ | *+ | + | ||
| Flt-1 (VEGF receptor 1), | + | *+ | *+/– | |||
| Flg (FGFR1) | *+++ | *++ | ||||
| MUC18 (melanoma-associated glycoprotein, CD146) | + | + | *+++ | *++ | + | |
| Collagen type I | + | + | + | |||
| Collagen type III | + | + | ||||
| Scleraxis | + | + | + | |||
| Osteo/odontogenic | ||||||
| ALP | + | + | *+++ | *++ | + | |
| BSP | +/– | *+ | *+ | + | + | |
| DSP | + | *+++ | *++ | |||
| MEPE (matrix extracellular phosphoglycoprotein) | + | *+++ | *++ | + | ||
| Cbfa1/Runx2 | + | *+ | *+ | |||
| Osteocalcin (OCN) | +/– | + | + | + | ||
| Osteonectin | +/– | + | ||||
| Osterix | + | + | + | |||
| Neurogenic | ||||||
| Nestin | *+ | *+ | + | |||
| βIII tubulin | + | + | ||||
| Tau | + | |||||
| MAP2 (microtubule-associated protein) | (+) | |||||
| GAD (glutamic acid decarboxylase), | + | (+) | ||||
| NeuN (Neuronal nuclear antigen), | + | (+) | ||||
| NFM (Neurofilament M) | − | + | *+/– | *+/– (+) | ||
| NSE (neuron-specific enolase) | + | |||||
| CNPase (glial markers 2’,3’-cyclic nucleotide 3’-phosphodiesterase) | + | + | ||||
| GFAP (glial fibrillary acidic protein), | + | + | + |
*
Comparison of relative expression levels of genes between DPSCs and SCAP. For other cells, + or—indicates only positive or negative expression; +, expression without stimulation; (+) expression after induction.
#
Markers listed do not exclude others not listed herein.