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. Author manuscript; available in PMC: 2010 Mar 2.
Published in final edited form as: Methods Cell Biol. 2008;89:275–308. doi: 10.1016/S0091-679X(08)00610-9

Table I.

Vibrational Frequencies (in cm−1) and Assignments of Peaks Found in the Spectra of Cells

3300 Amide A (N–H stretching mode, peptide linkage)
2950 CH3 antisymmetric stretching mode
2920 CH2 antisymmetric stretching mode
2880 CH3 symmetric stretching mode
2850 CH2 symmetric stretching mode
1735 >C=O stretching mode, ester linkage
1690–1620 Amide I (>C=O stretching mode, peptide linkage)
1570–1530 Amide II (C–N stretching mode, peptide linkage)
1468–1455 CH3/CH2 antisymmetric bending mode
1397 –COO symmetric stretching
1379 CH3 symmetric bending mode
1340–1240 Amide III (coupled N–H/C–H deformations)
1237 O–P=O antisymmetric stretching mode (PO2)
1150 C–O stretching, C–O–H bending modes (carbohydrates, mucin)
1083 O–P=O symmetric stretching mode (PO2)
1063 –CO–O–C symmetric stretching mode
1050 C–O stretching mode (carbohydrates, mucin)
1004 Phenylalanine ring breathing mode
968 C–O–P phosphodiester residue (DNA)