Fig. 7.

The cathepsin B inhibitor, E64d, prevents Cyt-dependent trypsinogen activation in ZG (A) and Mic (B) fractions. Assays were performed as for Fig. 3, but some samples were preincubated with E64d (1 mM) for 30 min. Control experiments (i.e., in the absence of E64d) were preincubated with 1:1 methanol-water, the vehicle for E64d. Data are normalized to amylase content and expressed as fold vs. ZG/Mic + Cyt + ATP; n = 3. Activation in Cyt alone was negligible (data not shown). *P < 0.05 vs. Cyt or Cyt/ATP condition.