Table 1. Percent zymogen activation in reconstituted system.
ZG | ZG/Cyt | ZG/Cyt/ATP | Mic | Mic/Cyt | Mic/Cyt/ATP |
---|---|---|---|---|---|
Trypsin activity | |||||
0 | 2.2±0.8 | 2.4±0.9 | 1.5±<0 | 5.0±1.0 | 4.2±0.8 |
Chymotrypsin activity | |||||
0 | 1.0±0.2 | 7.0±1.1 | 0.5±<0 | 6.0±3.1 | 10.0±3.8 |
Values are means ± SE. Activities are calculated as rfu/s per total protein content from each assay condition and expressed as a percentage of the total activated zymogen as determined in Fig. 2. ZG, zymogen granule-enriched fraction; Mic, microsomal fraction; Cyt, cytosol fraction.