Table 3. Effects of sucrose and KCl wash of particulate fractions on zymogen activation.
Wash Condition | ZG | ZG/Cyt | ZG/Cyt/ATP | Mic | Mic/Cyt | Mic/Cyt/ATP |
---|---|---|---|---|---|---|
Trypsin activity | ||||||
Sucrose | 35±12 | 94±12 | 100 | 4 | 94±4 | 100 |
KCI | 42±9 | 103±10 | 106 ± 9 | 3 | 98±3 | 104 |
Chymotrypsin activity | ||||||
Sucrose | 0 | 40±14 | 100 | 0 | 53±12 | 100 |
KCI | 0 | 28±17 | 111 ± 2 | 0 | 43±14 | 82 ± 17 |
Values are means of %activity ± SE. For ZG data, maximal activity (100%) is set as ZG/Cyt/ATP using sucrose-washed ZG fractions. For Mic data, maximal activity (100%) is set as Mic/Cyt/ATP using sucrose-washed microsomal fractions. Comparison of like conditions show no significant differences between sucrose or KCl.