Table 2.
PCR amplification of gfp transgene
| Experiment 1 |
||||||||
|---|---|---|---|---|---|---|---|---|
| Strain | Blood | Spleen | Liver | Thymus | Heart | Lung | Kidney | Brain |
| ROSA26- EGFP |
- | 814 | 849 | - | 4421 | 660 | 1530 | 698 |
| CD1 (wild- type) |
- | 0 | 0 | - | 0 | 0 | 0 | 0 |
| CAG | - | 1295 | 1774 | - | 3150 | 2121 | 3934 | 1644 |
| C57BL/6J (wild-type) |
- | 0 | 0 | - | 0 | 0 | 0 | 0 |
| Experiment 2 |
||||||||
|---|---|---|---|---|---|---|---|---|
| Strain | Blood | Spleen | Liver | Thymus | Heart | Lung | Kidney | Brain |
| ROSA26- EGFP |
376 | 983 | 463 | 646 | 2713 | 298 | 2451 | 793 |
| CD1 (wild- type) |
0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| CAG | 295 | 2640 | 1390 | 830 | 4539 | 595 | 7265 | 1341 |
| C57BL/6J (wild-type) |
0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Each row represents organs from a single mouse
Results expressed as pg of GFP DNA in 2500 pg genomic DNA
CAG: C57BL/6-Tg(ACTB-EGFP)10sb/J