Table 1.

Linearity, accuracy, and precision measurements for assay validation.

Linearity
Concentration (nM)	300	100	30	10
Mean (nM) n=6	311.42	97.22	31.38	10.10
Accuracy (% Recovery) n=6	103.81	97.22	104.6	101
Precision (% CV) n=6	5.30	5.21	10.05	13.17
Dilution Linearity	Sample 1	Dilution 1	Dilution 2	Dilution 3
Concentration (nM)	101.12	33.71	16.85	8.43
Mean (nM)	94.50	34.11	16.80	7.59
Accuracy (% Recovery)	93.45	101.19	99.70	90.04
Accuracy
Sample Recovery	Sample 1	Recovery 1	Recovery 2	Recovery 3
Mean (nM)	40.90	50.96	106.45	334.94
HepC-25 Added (nM)		10	60	300
Recovered (nM)		10.06	65.55	294.04
Recovery (%)		101	109	98
Analyte Interference	Sample 1	Interference 1	Interference 2
Mean (nM)	101.12	114.74	108.89
NaCl Added (M)	0	0.25	1.0
Interference (nM)		13.63	7.78
Precision
Inter-spot/Inter-day	Measurements	Sample 1	Sample 2	Sample 3
Day 1	Mean (nM)	106.05	25.33	10.30
	Precision (%CV)	5.75	11.28	20.47
	n	6	6	4
Day 2	Mean (nM)	110.77	30.23	11.97
	Precision (%CV)	7.38	11.41	10.04
	n	6	6	6
Day 3	Mean (nM)	101.79	29.76	11.78
	Precision (%CV)	9.43	12.42	23.63
	n	6	6	6
Total	Mean (nM)	106.20	28.44	11.48
	Precision (%CV)	7.92	13.65	18.38
	n	18	18	16