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. Author manuscript; available in PMC: 2011 Mar 19.
Published in final edited form as: J Mol Biol. 2010 Jan 15;397(1):278–289. doi: 10.1016/j.jmb.2010.01.017

Figure 6.

Figure 6

Transcriptional activity of Elf3 and its point mutants. (A) F9-differentiated cells were transiently transfected with the promoter/reporter construct mTGFβ-RII (−108/+56) and 1 or 3 μg of an expression plasmid for flag-tagged Elf3 with either the wild-type (WT) or indicated point mutant sequence. CAT reporter gene activity was measured and normalized as described in Materials and Methods. Independent clones of plasmids for the N357A and W361A mutants gave similar results and this experiment was repeated twice to verify the intermediate effects of the R349A clone. (B) Western blot analysis was performed using cell nuclear extracts from 293T cells transfected with the expression plasmids used in part (A) and an antibody against the N-terminal flag tag to visualize relative expression levels.