Figure 3. Effect of transient expression in T-Rex™ HeLa cells of CaMKKβ, or stable expression of LKB1, on the phosphorylation and activity of AMPK and ARKs.

(A) Endogenous AMPK activity; (B) Western blot analysis of duplicate cell lysates shown in (A); (C–F) activities of endogenous SIK1, SIK2, SIK3 and NUAK2. Values are means±S.D. for duplicate cell incubations. *P<0.05, **P<0.01 and ***P<0.001, significantly different from control without CaMKKβ or LKB1.