Figure 2.

Localization and chromosome dimer resolution activity of FtsK_LC chimaeras. (A) Schematic view of septum assembly. The protein icons are ordered from left to right according to the commonly accepted assembly sequence. OM, outer membrane; IM, inner membrane. (B) Schematic representation of the FtsK_LC chimaeras as in Figure 1B. (C) Localization of FtsK_LC chimaeras fused at their C-terminal ends to YFP and of full-length FtsK fused at its N-terminal end to GFP in ftsK⁺ cells. A similar localization pattern was observed in ftsA* ftsK⁻ and in ftsK_LC⁻ cells (data not shown). (C) Pictures of ftsK⁺ cells are shown because of their simpler cell division phenotype. Scale bars (white bars), 1 μm. (D) Growth competition between ftsA^* ftsK⁻ and ftsA^* ftsK⁻ Δdif cells. The graph of a typical result is shown. f, mean and standard deviation of the growth advantage given by the ectopic production of each FtsK_LC chimaera (result from three independent experiments).