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. 2009 Dec 10;29(3):606–618. doi: 10.1038/emboj.2009.369

Figure 3.

Figure 3

NAF-1 does not affect BIK-initiated apoptosis, but influences BIK-induced autophagy in the absence of caspase activation. (A) H1299 neo and HA-BCL-2b5 cells treated with control (CTRL) or NAF-1 shRNA were either mock infected or infected with Ad-BIK in the absence or presence of 50 μM zVAD-fmk. Cell lysates were analysed by immunoblot. (B) Caspase activity was measured using the fluorescent substrate DEVD-AMC. The results represent the average±s.d. of three independent experiments. (C) Prolonged BIK expression and caspase inhibition induces autophagy, which is enhanced by knockdown of NAF-1. H1299 neo and HA-BCL-2b5 cells treated with CTRL or NAF-1 shRNA were infected with Ad-BIK in the presence of zVAD-fmk for the indicated periods of time. Cell lysates were analysed by immunoblot. Levels of LC3 II were normalized to actin levels by densitometry analysis. Graph depicts normalized LC3 II levels of each lane.