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. 2009 Dec 10;29(3):606–618. doi: 10.1038/emboj.2009.369

Figure 4.

Figure 4

NAF-1 knockdown results in increased incidence and intensity of starvation-induced Beclin 1-dependent autophagy. (A) Effect of NAF-1 knockdown on starvation-induced autophagy. H1299 cells infected with CTRL or NAF-1 shRNA were starved for 4 h in EBSS with DMSO (vehicle) or Baf A1 (100 nM). Cell lysates were analysed by immunoblot. (B) Representative images of GFP-LC3 staining in H1299 GFP-LC3 cells transfected with LUC or NAF-1 siRNA, untreated and starved. Scale bar represents 10 μm. (C) Quantification of autophagy is expressed as the percentage of GFP-LC3-expressing cells displaying punctate GFP-LC3. A minimum of 100 cells per sample were counted; results represent the average±s.d. of three independent experiments. Cell lysates were analysed by immunoblot.