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. 2009 Dec 17;29(3):559–573. doi: 10.1038/emboj.2009.370

Figure 8.

Figure 8

Modulation of Trb3 and miR-24 expression in a rat PAH model (A). qRT–PCR analysis of lung samples from rats after 17-day hypoxia or normoxia treatment. Relative expression of Trb3, SMA, CNN, and SM22 (top panel); or miR-24, miR-221, and miR-100 (bottom panel) was plotted by normalizing the level of expression of normoxia samples as 1 (means±s.e.m., n=3; *P<0.001). (B) Histological examination of PAs from rats after 17-day hypoxia or normoxia treatment with anti-SMA, anti-Smad1, anti-phospho Smad1/5 (Pi-Smad1/5), or anti-Trb3 antibodies (brown, × 400) and by haematoxylin staining (blue, × 400). The media (M) and adventitia (A) of the PAs are indicated. (C) Sections of PAs were probed with either anti-miR-24 (top two panels) or control (bottom panel) conjugated to FITC (green). Cells were counterstained with DAPI (blue). The media (M) and adventitia (A) of the PAs are indicated. Relative expression of miR-24 was quantitated using the ImageJ software and imaged (n=3; *P<0.001).