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. 2009 Dec 17;29(3):632–642. doi: 10.1038/emboj.2009.380

Figure 2.

Figure 2

The isolation and characterization of PAMP-binding fragments released on proteolytic activation of Hb-POX cycle. Pull-down of proteolysed fragments by PAMP-Sepharose. (A) The production of free radicals (represented by superoxide) in the fragments bound to the PAMP-Sepharose (denoted as PAMP:metHb:protease; ‘S', ‘E' and ‘K' are subtilisin A, elastase and proteinase K, respectively) was detected by CLA-CL assay. (B) The eluted metHb fragments were resolved on Tris–Tricine SDS–PAGE (10%), and the proteins were identified by MS. (C) The MS-inferred region of the isolated PAMP-binding fragments. The modelled domain structures of the respective fragments are indicated. Co-migration of fragments covering both N-/C-terminal sequences was detected as Hbβ-F2. Only one peptide mass fingerprint was identified for Hbα-F1.