Figure 4.

Invariance of the proximal–distal contact. (A) The 8 Å resolution map of the D6 coat (Fotin et al, 2004a), with the model of corresponding heavy-chain segments. The view is in a direction tangential to the surface of a coat, with the exterior of the lattice above and the interior below. (B) Corresponding map and model for the Hsc70:auxilin:clathrin complex. (C) Superposition of the two, with the map from the uncomplexed coat in blue (as in A) and the map from the ternary complex in brown (as in B). The two maps were positioned to optimize agreement in the proximal-leg region, and the excellent superposition of the distal-leg maps shows that the interface does not shift when the ligands distort the coat.