Figure 4.

Transferrin uptake is attenuated when Bves is disrupted. (A) MDCK and Bves118 cells internalized labelled transferrin for 5, 10, or 20 mins. Transferrin uptake, as measured by the MFI, was significantly decreased in Bves118 cells at all time points. (B) When normalized with COMO values (100%), Bves MO- and VAMP3 (V3) MO-treated caps were impaired in internalization of labelled transferrin/μg of total protein. Transferrin uptake is restored when Bves MO or V3 MO are co-injected with rescue Bves (Bves MOR) or VAMP3 (V3 MOR) RNAs.