Cumulative lysozyme release from LbL-coated 3% agarose hydrogel, triggered by physiological pH, a) with varying stacking order of the polymers within a multilayer but the same number of cumulative bilayers (as shown in Scheme 1b–d) and b) with two-component assembly of lysozyme and PAA, two-component assembly of lysozyme and PEG, and three component assembly of PAA, PEG and lysozyme (as shown in Scheme 1f, e, and b respectively).