Fig. 19.
F-actin accumulation into cell–substrate anchoring structure. F-actin was visualized by expressing GFP-coronin, an actin-binding protein and imaged by spinning-disk laser confocal microscope at video rate. Pixel intensity was exhibited on the z-axis and then converted into color using an algorithm as shown by the color bar. The image sequence represents the F-actin dynamic every 30 s [40].