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Diagram of the dHAND-Cre transgene. A 7.4-kb fragment of the dHAND upstream region, containing enhancers required for pharyngeal arch and cardiac expression, was fused to a Cre cDNA containing nuclear localization and SV40 polyadenylation sequences. A synthetic intron resides in the 5′ end of the Cre cDNA to aid transgene expression. Bg, BglII; Kp, KpnI; Sf, SfiI; Xh, XhoI.
